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RG011,OD104,皮克林/Pickering柱后衍生--钠离子交换柱法测氨基酸

发布者:凯迈科  发布时间:2019-03-06  转自:凯迈科  查看:次  【

文章摘要: RG011,OD104,皮克林/Pickering柱后衍生--钠离子交换柱法测氨基酸
RG011,OD104,皮克林/Pickering柱后衍生--钠离子交换柱法测氨基酸
OPA 用于衍生分析氨基酸是1971年首次提出。图 1 为 OPA 与一级氨基酸的反应方程式。

 
室温下, OPA (Pickering O120)巯基乙醇(Pickering 3700-2000)环境中与一级氨基酸于 1 min 内即可生成具有强荧光的异吲哚衍生产物。OPA 本身不发荧光的特性使其在色谱分离时不形成干扰峰。OPA 只能与经次氯酸钠等氧化剂氧化开环后的二级氨基酸反应,也可与 FMOC - Cl 联用衍生二级氨基酸。OPA 衍生法因样品制备简单、衍生反应迅速、灵敏度高及易于实现自动化操作等优点而成为柱前衍生反相高效液相色谱法中使用最广泛的方法之一。
OPA 衍生法(用于检测一级氨基酸)
试剂: 300 mg OPA (Pickering O120), 2 g Thiofluor™ (Pickering 3710-2000), 3 mL 30 % Brij® 35 溶于 950 mL OPA稀释液(Pickering OD104)中
反应器: 45 °C, 0.15 mL
衍生试剂流速: 0.3 mL/min
检测器: 荧光检测器
λex= 330 nm, λem=465 nm


相关产品目录

钠离子交换柱及保护柱
1154150T High-efficiency Sodium Cation-exchange for protein, collagen and oxidized feed hydrolysates
Column 4.0 × 150 mm & 1700-0070 amino acid test mixture
1154110T 30-minute High-efficiency Sodium Cation-exchange Column 4.6 × 110 mm & 1700-0070 amino
acid test mixture
1193250 Standard Sodium Cation-exchange Column 3.0 × 250 mm & 1700-0070 amino acid test mixture
1700-3102 Cation-exchange GARD™ Assembly: Includes holder and 2 replaceable GARDs™
1700-3101 Replacement Cation-exchange GARDs™ (2/PK)
1700-3100 GARD™ Holder
30 分钟快速分析包  
0352-0058 30-MIN HIGH-EFFICIENCY PROTEIN HYDROLYSATE KIT:
1154110T 30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-
0070 amino acid test mixture
1700-3102 Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARDs™
Na220 Sodium Diluent, pH 2.20, 4 x 250 mL
Na315 Sodium Eluant, pH 3.15, case of 4 ( 950 mL/bottle)
Na425 Sodium Eluant, pH 4.25, 4 x 950 mL
Na640 Sodium Eluant, pH 6.40, 4 x 950 mL
RG011 Sodium Column Regenerant, 950 mL
T200 TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506H Sodium Calibration Standard, for protein hydrolysate, 0.25 µmole/mL, 5 mL
0352-0059 KIT IDENTICAL TO 0352-0058 WITH ITEMS BELOW REPLACING T200
OD104 OPA Diluent, 4 x 950 mL
O120 o- Phthalaldehyde, 5 g
3700-2000 Thiofluor™, 10 g (2 each per kit)
0352-0062 30-MIN HIGH-EFFICIENCY COLLAGEN HYDROLYSATE KIT:
1154110T 30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-
0070 amino acid test mixture
1700-3102 Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARDs™
Na220 Sodium Diluent, pH 2.20, 4 x 250 mL
Na315 Sodium Eluant, pH 3.15, case of 4 ( 950 mL/bottle)
Na425 Sodium Eluant, pH 4.25, 4 x 950 mL
Na640 Sodium Eluant, pH 6.40, 4 x 950 mL
RG011 Sodium Column Regenerant, 950 mL
T200 TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506C Sodium Calibration Standard, for collagen hydrolysate, 0.25 µmole/mL, 5 mL
0352-0063 KIT IDENTICAL TO 0352-0062 WITH ITEMS BELOW REPLACING T200
OD104 OPA Diluent, 4 x 950 mL
O120 o- Phthalaldehyde, 5 g
3700-2000 Thiofluor™, 10 g (2 each per kit)
 
0352-0021 30-MIN HIGH EFFICIENCY OXIDIZED FEED HYDROLYSATE KIT:
1154110T 30-Minute Sodium Cation-exchange Column 4.6 x 110 mm & 1700-0070 amino acid test mixture
1700-3102 Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARDs™
Na220 Sodium Diluent, pH 2.20, 4 x 250 mL
Na270 Sodium Eluant, pH 2.80, 4 x 950 mL
Na425 Sodium Eluant, pH 4.25, 4 x 950 mL
Na640 Sodium Eluant, pH 6.40, 4 x 950 mL
RG011 Sodium Column Regenerant, 950 mL
T200 TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
1700-0155 Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25 µmole/mL, 5 mL
0352-0022 KIT IDENTICAL TO 0352-0021 WITH ITEMS BELOW REPLACING T200
OD104 OPA Diluent, 4 x 950 mL
O120 o- Phthalaldehyde, 5 g
3700-2000 Thiofluor™, 10 g (2 each per kit)
55 分钟高效分析包  
0352-0031 HIGH-EFFICIENCY 55-MIN PROTEIN HYDROYSIS KIT:
1154150T Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102 Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARDs™
Na220 Sodium Diluent, pH 2.20, 4 x 250 mL
Na315 Sodium Eluant, pH 3.15, 4 x 950 mL
Na740 Sodium Eluant, pH 7.40, 4 x 950 mL
RG011 Sodium Column Regenerant, 950 mL
T200 TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506H Sodium Calibration Standard, for protein hydrolysate, 0.25 µmole/mL, 5 mL
AO31FH KIT IDENTICAL TO 0352-0031 WITH ITEMS BELOW REPLACING A T200
OD104 OPA Diluent, 4 x 950 mL
O120 o- Phthalaldehyde, 5 g
3700-2000 Thiofluor™, 10 g (2 each per kit)
0352-0032 HIGH-EFFICIENCY 55-MIN COLLAGEN HYDROLYSATE KIT
1154150T Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102 Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARDs™
Na220 Sodium Diluent, pH 2.20, 4 x 250 mL
Na315 Sodium Eluant, pH 3.15, 4 x 950 mL
Na740 Sodium Eluant, pH 7.40, 4 x 950 mL
RG011 Sodium Column Regenerant, 950 mL
T200 TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506C Sodium Calibration Standard, for collagen hydrolysate 0.25 µmole/mL, Proline and Hydroxyproline, 1.25
µmole/mL, 5 mL
AO32FC KIT IDENTICAL TO 0352-0032 WITH ITEMS BELOW REPLACING A T200
OD104 OPA Diluent, 4 x 950 mL
O120 o- Phthalaldehyde, 5 g
3700-2000 Thiofluor™, 10 g (2 each per kit)
 
0352-0017 60-MIN OXIDIZED FEED HYDROLYSATE KIT:
1154150T Sodium Ion-exchange Column, 4.0 x 150 mm (with test mixture 1700-0070)
1700-3102 Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARDs™
Na220 Sodium Diluent, pH 2.20, 4 x 250 mL
Na270 Sodium Eluant, pH 2.80, 4 x 950 mL
Na740 Sodium Eluant, pH 7.40, 4 x 950 mL
RG011 Sodium Column Regenerant, 950 mL
T200 TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
1700-0155 Sodium Calibration Standard, for oxidized feed hydrolysate, 0.25 µmole/mL, 5 mL
0352-0019 KIT IDENTICAL TO 0352-0017 WITH ITEMS BELOW REPLACING T200
OD104 OPA Diluent, 4 x 950 mL
O120 o-Phthalaldehyde, 5 g
3700-2000 Thiofluor™, 10 g, (2 each per kit)
60 分钟标准分析包  
0352-0030 STANDARD 60-MIN PROTEIN HYDROLYSATE KIT
1193250 Sodium Ion-exchange Column, 3.0 x 250 mm (with test mixture 1700-0070)
1700-3102 Cation-exchange GARD™ assembly: Holder w/ 2 replaceable GARDs™
Na220 Sodium Diluent, pH 2.20, 4 x 250 mL
Na328 Sodium Eluant, pH 3.28, 4 x 950 mL
Na740 Sodium Eluant, pH 7.40, 4 x 950 mL
RG011 Sodium Column Regenerant, 950 mL
T200 TRIONE® Two-part Ninhydrin Reagent, prepares 4 x 900 mL (12-month shelf life)
012506H Sodium Calibration Standard, for protein hydrolysate, 0.25 µmole/mL, 5 mL
AO30SH KIT IDENTICAL TO 0352-0030 WITH ITEMS BELOW REPLACING T100C
OD104 OPA Diluent, 4 x 950 mL
O120 o-Phthalaldehyde, 5 g
3700-2000 Thiofluor™, 10 g, (2 each per kit)
标准品&测试品  
012506C Collagen Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
L,L & allo-Hydroxylysine 4-trans-L-Hydroxyproline(1.25)  
012506H Protein Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
Leucine Lysine  
1700-0155 Oxidized Feed Hydrolysate Standard, in 0.2 N Sodium citrate buffer pH 2.20, 5 mL
Leucine Lysine  
1700-0070 Amino Acid Test Mixture Concentration 0.25 µmole/mL in 0.01 NHCl, 5.0 mL
Cysteic Acid Threonine Serine  

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